The Leonard lab investigates the mechanisms by which signals are transduced in the cell into appropriate downstream effects. We use a wide variety of biochemical, biophysical and structural biology tools, complemented with cell biology to understand how cells regulate the transmission of information at the molecular level. By understanding the mechanisms by which these signals are transduced and propagated, we can better understand why and how things go wrong in disease.
For more information on the Leonard lab please visit the lab’s group page.
The Querques lab studies the molecular mechanisms by which mobile genetic elements (MGEs) move between and within genomes. Our research aims not only to understand the biological impacts of MGEs on genome remodeling, but also to inform their development as genome engineering tools. We employ an integrative approach, combining structural biology techniques (chiefly cryo-electron microscopy and X-ray crystallography) with biochemical and biophysical methods, and biotechnological approaches (cell-based functional assays, protein design and genome editing experiments). More information can be found on the Querques lab’s group page.
We are looking for a highly-motivated scientist to support our research activities. Your responsibilities would include:
Working closely with team members to support experiments in biochemistry, molecular biology, or related areas
Using and understanding a wide variety of molecular and cell biology techniques – such as cloning, protein/DNA electrophoresis, protein expression and purification, cell culture maintenance and transfection protocols, preparing reagents and common stocks (including competent cells and enzymes)
Identifying and applying improvements to shared resources and protocols
Training other lab members in the operation of specialized equipment and provide instruction and guidance on the use of general equipment and standard operating procedures
Actively participating in group meetings and journal clubs, staying up to date on literature in our research areas and consulting with other lab members on projects
Lab Administration (50%)
Gathering and placing lab orders, collect receipts and monitor expenditures
Management of lab stocks, databases, records and inventory
Acting as point of contact for communications with technicians/sales representatives
Acting as Radiation Safety Officer and responsible person for toxic waste disposal and routine refilling of liquid nitrogen tanks
Assisting with shipment of biological samples, reagents and equipment
Assisting with equipment management, maintenance and running of routine controls on equipment (in particular Åkta Pure FPLC systems)
Maintaining insect and mammalian cell stocks
Maintaining mammalian cell culture room (BSL-2) and dedicated equipment. Previous experience with working at BSL‐2 is desired, but not strictly required
Assisting with the onboarding of new team members
Assisting with the management of grant spending and budgets
Hold a Master’s degree in biochemistry, molecular biology or a related discipline
Have at least 2 years hands-on experience in a research lab. Experience with recombinant protein expression (including insect and mammalian cell expression), protein purification, biophysical characterization (including structural biology techniques) and mammalian cell culture maintenance is highly desired
Be self-motivated and able to perform complex tasks effectively and independently
Have excellent oral and written communication skills in both English and German
Have exemplary organizational skills and attention to detail
Be able to work effectively in a team.
who is excited by science
who is fascinated by molecular mechanisms and genome engineering applications
who is creative, critical, and communicative
Salary corresponds to the University “Kollektiv-Vertrag” according to qualification and experience. The Research Technician will be affiliated with both the Medical University of Vienna (50%, Leonard lab) and the University of Vienna (50%, Querques lab). The application deadline is February 28, 2023.