A) Analysis of plant ubiquitin ligases via a synthetic biology approach in the budding yeast S. cerevisiae. We are analyzing protein turnover in plants that depends on amino-terminal degradation signals. Expression of the involved enzymes in yeast is part of mechanistic studies on these turnover pathways.
B) Construction of expression vectors for novel reporter proteins (tandem fluorescent timer constructs). The vectors shall serve for analysis of half-life and subcellular localization of fused proteins. The constructs shall be tested in the model plant, A. thaliana.
Focus of the Bachmair lab lies on the biochemical and cell biological analysis of protein turnover driven by amino-terminal degradation signals. The biological context is how plants respond to environmental stimuli such as heat, flooding or salt stress. Additional information can be found on the Bachmair lab web site (contact address below).
Requirements: Finished study (B. Sc.) in Molecular Biology, Biochemistry or related, 45 ECTS accomplished in the Master module
Duration: ca. 12 months
Payment: acc. to FWF rates
Univ.-Prof. Dr. Andreas Bachmair
Max Perutz Labs,
Dept. of Biochemistry and Cell Biology, University of Vienna
Rm. 5110, Dr.-Bohr-Gasse 9, A-1030 Wien
Millar, A. H., Heazlewood, J. L., Giglione, C., Holdsworth, M. J., Bachmair, A., Schulze, W. X. (2019) The scope, functions, and dynamics of posttranslational protein modifications. Annu Rev Plant Biol 70, 119-151.
Vicente, J., Mendiondo, G. M., Pauwels, J., Pastor, V., Izquierdo, Y., Naumann, C., Movahedi, M., Rooney, D., Gibbs, D. J., Smart, K., Bachmair, A., Gray, J. E., Dissmeyer, N., Castresana, C., Ray, R. V., Gevaert, K., Holdsworth, M. J. (2019) Distinct branches of the N-end rule pathway modulate the plant immune response. New Phytol 221, 988-1000
Tomanov, K., Nehlin, L., Ziba, I., Bachmair, A. (2018) SUMO chain formation relies on the amino-terminal region of SUMO conjugating enzyme and has dedicated substrates in plants. Biochemical J 475, 61-68.
Nukarinen, E., Tomanov, K., Ziba, I., Weckwerth, W., Bachmair, A. (2017) Protein sumoylation and phosphorylation intersect in Arabidopsis signaling. Plant J. 91, 505-517.