Model substrates and known substrates from plants shall serve to compare the activity of three different ligase types in the heterologous host. Expression of the involved enzymes in yeast is part of mechanistic studies on this turnover pathway. In addition, tagged ligases shall be affinity-purified from yeast cells for Cryo-EM structural characterization.
If time allows, expression vectors for novel types of reporters, tandem fluorescent timer (tFT) proteins, can be constructed. The tFT reporter proteins serve to investigate tissue-specific turnover and subcellular localization of metabolically unstable proteins in plants (test by transient expression in tobacco leaves, then stable expression in A. thaliana).
Focus of the Bachmair lab lies on the biochemical and cell biological analysis of protein turnover driven by amino-terminal degradation signals. The biological context is how plants respond to environmental stimuli such as heat, flooding or salt stress. Additional information can be found on the Bachmair lab page.
Finished study (B. Sc.) in Molecular Biology, Biochemistry or related, 45 ECTS accomplished in the Master‘s module.
ca. 12 months
acc. to FWF rates
Univ.-Prof. Dr. Andreas Bachmair
Max Perutz Labs, Dept. of Biochemistry and Cell Biology, Univ. Wien
Rm. 5110, Dr.-Bohr-Gasse 9, A-1030 Wien
Winter, N., Novatchkova, M., Bachmair, A. (2021) Cellular control of protein turnover via modification of the amino terminus. Int J Molec Sci 22, 3545.
Millar, A. H., Heazlewood, J. L., Giglione, C., Holdsworth, M. J., Bachmair, A., Schulze, W. X. (2019) The scope, functions, and dynamics of posttranslational protein modifications. Annu Rev Plant Biol 70, 119-151.
Vicente, J., Mendiondo, G. M., Pauwels, J., Pastor, V., Izquierdo, Y., Naumann, C., Movahedi, M., Rooney, D., Gibbs, D. J., Smart, K., Bachmair, A., Gray, J. E., Dissmeyer, N., Castresana, C., Ray, R. V., Gevaert, K., Holdsworth, M. J. (2019) Distinct branches of the N-end rule pathway modulate the plant immune response. New Phytol 221, 988-1000