The goal: identify the turn motif kinase among >500 human protein kinases.
Akt is an essential protein kinase with roles in growth and proliferation, cell survival and metabolism. Activated by growth factors and the immediate effector of PI3K signaling, it is hyperactivated in >50% of human cancers and a therapeutic target of anti-cancer drugs.
Akt is regulated by phosphorylation of three sites in its kinase domain, one of which is called the turn motif. The turn motif is peculiar not just to Akt, but to 25 other protein kinases, for whom it regulates protein stability/turnover as well as intrinsic catalytic activity. The identity of the kinase that phosphorylates the turn motif, however, is unknown. This gap in our knowledge precludes a full understanding of the mechanisms that control the cellular levels and catalytic activities of these kinases.
This project will take an unbiased approach using in vitro biochemistry tools to identify the responsible turn motif kinase. The candidate will design, optimize, and utilize one or more biochemical assays to identify the kinase in mammalian cell lysates. The project will involve assay design, molecular biology, protein expression and purification, chromatographic purification of complex mixtures, assay development, and mass spectrometry.
Be excited by science
Be creative, critical, and communicative
Hold a Bachelor’s degree in biochemistry, chemistry or molecular biology
Have practical experience in molecular biology
Apply now by sending your CV and motivation letter to Thomas Leonard (email@example.com).
The Max Perutz Labs are a research institute established by the University of Vienna and the Medical University of Vienna to provide an environment for excellent, internationally recognized research and education in the field of Molecular Biology. Dedicated to a mechanistic understanding of fundamental biomedical processes, scientists at the Max Perutz Labs aim to link breakthroughs in basic research to advances in human health. The Max Perutz Labs are located at the Vienna BioCenter, one of Europe’s hotspots for Life Sciences, and host around 50 research groups, involving more than 450 scientists and staff from 40 nations.