The Monoclonal Antibody Facility generates mouse monoclonal antibodies against any custom antigen of choice, including short peptides, recombinant proteins, post-translational modifications, or small compounds. Our services comprise immunizations of mice (Balb/c), screening of mouse sera by Western blot and/or ELISA, fusion of splenocytes and screening of fusion clones, as well as the production of the resulting monoclonal antibodies. We put a particular emphasis on the robust and detailed antibody validation in close collaboration with our customers. In contrast to other commercial sources, which offer peptide-ELISA positive clones, we validate every antibody for the recognition of full-length antigens by Western blotting. In addition, we strongly encourage our customers to screen in their own preferred applications for the desired antibodies at the earliest possible stage, i.e. already in the mouse immune sera. Ourfacility was established in 2009. Since then, we have carried out over 130 projects for more than 50 customers, and we have generated a total of about 200 western blot validated monoclonal antibodies.
Please contact stefan.schuechner@univie.ac.at
Tel. +43-1-4277-61731
Max Perutz Labs Main Building
Dr. Bohr Gasse 9, 1030 Vienna
Level 2 - Room 2.127
Machine Learning Enabled Pooled Optical Screening for Target Discovery in Human Cellular Disease Models
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Unraveling the Complexity of Crossover Regulation in C. elegans
Dynamics of 3D Genome Structure and Function
How superworms can help to solve our plastic waste crisis
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New players in an old pathway: biology of methanogens of the TACK superphylum
Targeting a gerozyme to reverse muscle aging and increase healthspan
Shaping morphogen gradients: from molecules to tissues and back
Title to be announced
Studying stressed cells by in situ structural biology
The role of non-canonical double strand break repair pathways in genome stability and diversity
Exploring Microbial Resilience: Unravelling Escherichia coliand#x27;s Stress Response at the Level of Protein Synthesis
Arbuscular mycorrhiza development and function
Deep homology and deep diversity: Evolving genetic toolkits for making and sensing light
The evolution of cell type identity and tissue microecology at the fetal-maternal interface
The unanticipated roles of PICIs and phages in bacterial evolution
Chemical targeting of subcellular protein localization
Origin and diversification of gut-derived organs in chordates
Job's Dilemma for the Genome: Why Bad Things Happen to Good Chromosomes
Connections between carbon and nitrogen cycling in the ocean
Understanding how the DNA-loop-extruding protein complex Condensin folds a chromatinized genome into mitotic chromosomes
DrugMap: A quantitative pan-cancer analysis of cysteine ligandability
From Roads to Rivers? Occurrence and environmental fate of tire and road wear particles and of tire-related chemicals
FENS 2024 Satellite event: Home cage behavior monitoring at the interface of animal welfare and neuroscience
A mechanism for protein complex regulation at the mitochondrial surface
Striking physiology and cell biology of (marine) environmental microorganisms
Mechanisms controlling maintenance of cohesin dependent loops
Title to be announced