Light microscopes are essential tools in modern life science labs, enabling researchers to look beyond what can be seen with the naked eye. The Light Microscopy Facility is a cornerstone for cutting-edge research at the Perutz. Compared to the human eye, which can resolve objects 1/10th of a millimeter apart, state-of-the-art microscopes can enhance resolution by 500 times, down to ~200 nanometers, while even more specialized super-resolution technologies can achieve a resolution in the tens of nanometers. This enables researchers to study cells and subcellular structures. This remarkable capability makes light microscopy an indispensable window into the living cell.
From imaging cells…
The facility offers a diverse range of equipment, including confocal, spinning disk, and fast widefield microscopes, enabling the investigation of a wide variety of biological samples. Josef Gotzmann, head of the Light Microscopy facility, explains: “Our users primarily work with animal cells, which are about 10 to 50 times bigger than bacteria, but also with whole organisms such as yeast, fish, and worms.” Beyond capturing still images, there is an increasing demand for live imaging to observe biological processes in real-time. Since biological samples naturally lack color, the facility enables the visualization of stained samples by immunofluorescence, while an ever-expanding palette of fluorescent proteins allows users to visualize their favorite protein in live cells using one of the facilities’ advanced fluorescent microscopes. The team offers 24/7 accessibility to five in-house microscopes to support any research schedule; personalized training as well as troubleshooting ensure optimal user-oriented operation of the instruments. Another crucial post-acquisition step in the research workflow is image analysis – transforming colorful images into biologically meaningful data. Imaging specialist Nicholas Wedige supports users throughout the experimental process, helping them to analyze and interpret their data within the specific biological context.
…to tissues
The Histology facility at the Perutz supports the imaging of tissues and organoids, providing insights into how cells interact, function, and respond to various conditions. The facility offers a wide range of histological services, from tissue processing and embedding to sectioning and staining, tailored to support diverse research projects. Facility Head Irmgard Fischer says: “The Histology facility is extremely versatile, meeting the diverse requirements of users.” This enables high-quality research in developmental biology.