Confocal laser scanning microscope, inverted. Equipped with 3 PMTs, transmitted light PMT, and Airyscan 1 detector for super-resolution. Spectral imaging via sequential acquisition (PMTs only), motorized Piezo stage. Allows acquisition of time series, z stacks, tile scans, bleaching protocols and complex workflows. Widefield illumination: halogen lamp, X-Cite short-arc lamp.
EC Plan-Neofluar 10x/0.3 M27
LD LCI Plan-Apochromat 25x/0.8 Imm Corr M27 (immersion correction oil/glycerol/water)
Plan-Apochromat 40x/1.4 Oil DIC M27
Plan-Apochromat 63x/1.4 Oil DIC M27 - for standard confocal imaging
Plan-Apochromat 63x/1.4 Oil DIC M27 - for Airyscanning
alpha Plan-Apochromat 100x/1.46 Oil DIC M27 - for Airyscanning
405 nm laser diode (30 mW)
458, 477, 488, 514 nm Argon laser (25 mW)
561 nm DPSS laser (20 mW)
633 nm HeNe laser (5 mW)
Inverse point scanning confocal microscope with 2 PMT channels, transmitted light PMT and motorized stage. Spectral imaging via sequential acquisition, onboard software for tiling & stitching, macro for complex workflows. Widefield illumination: halogen lamp, CoolLED pE-2
EC Plan-Neofluar 10x/0.3
LD LCI Plan-Apochromat 25x/0.8 Imm Corr (oil, glycerol, water)
Plan-Apochromat 40x/1.3 Oil DIC
Plan-Apochromat 63x/1.4 Oil DIC
405 nm (5 mW)
488 nm (10 mW)
555 nm (10 mW)
639 nm (5 mW)
Two image processing workstations (room 6.508, Max Perutz Labs main building) providing stand-alone microscope software (Olympus cellSens, ZEN 2012, Visiview) and professional software packages for deconvolution and 3/4D image processing (SVI Huygens, softWoRx suite)
HIVE acquisition server for remote saving of images (access instructions)
Zeiss Stereomicroscope Stemi2000 (transmitted/incident light; room 1.320)
Leica Stereomicroscope S6E (transmitted/incident light; room 1.318)
BSL2 Tissue Culture Hood (room 1.219)
BSL1 Tissue Culture (room 1.219, hood, microscope, incubator, freezer & fridge)
Five microscopes (one in each floor + teaching microscope) in the MPL main building have been upgraded to state-of-the-art technology. They are fully motorized, host high-end CCD cameras and are fully equipped for fluorescence/brightfield imaging. Irmgard Fischer is the responsible person for these microscope. She helps in training and troubleshooting and maintenance/service.
Irmgard Fischer; Facility Technician; Rooms 4.621/5.506; Tel. ext. 52866; mobile:0660/7681381; Irmgard.firstname.lastname@example.org
2nd floor (room 2305) - Axiovert 200M
3rd floor (room 3517) - Axio Observer Z1
4th floor (room 4407) - Axio Imager Z2
5th floor (room 5423) - Axio Imager M2
Teaching (room 5519) - Axio Observer Z1
Potential trainees must provide an organized experimental strategy to discuss with the facility staff and have already own samples for an individualized training session.
Attend the Introductory Lecture Biooptics including laser safety instructions.**
Organize a training unit with the facility staff – training units will be split into "how to do" and "optimize my sample" sessions (on separate days).
Fill in and sign the Training Confirmation on usage of microscopes/user fee regulations (trainee and group leader) and bring it to your training session.
Before you attend the sessions, please download our General Administrative Rules and read them thoroughly!
*Optional: facility personnel evaluates potential applicability with user specific samples, if selection of the proper microscope system remains unclear.
**1.) and 2.) may be switched.
Lectures always take place in the seminar rooms in VBC5, level E1. Lecture dates are announced regularly. To register, login to the MFPL Intranet, select "Booking System" and then ”Lecture and Training Registration".
Please contact the facility staff: email@example.com
VBC5 - Structural and Computational Biology Dept., Level E1
Campus-Vienna-Biocenter 5, 1030 Vienna
Office: room 1.618 (phone ext. 61672)
Microscopes 1: room 1.223 (phone ext. 61678)
Microscopes 2: room 1.320 (phone ext. 61677)
Microscopes 3: room 1.318 (phone ext. 61679)
Microscopes 4: room 1.723 (CIBIV; phone ext. 61675)
Tissue Culture: room 1.219 (phone ext. 52247)
Image Processing workstations:
Max Perutz Labs main building,
Dr.-Bohr-Gasse 9, 1030 Vienna
6th floor, room 6.508.
November 20, 2018
The new deconvolution widefield fluorescence microscope from GE - Deltavision ULTRA - has been installed after thorough testing and is now ready for training and use. It is a refurbished, technically upgraded version of our existing Deltavision microscope. The new technical features include a new acquisition software (Acquire Ultra), a hardware autofocus, a fast and sensitive sCMOS camera, a 60x silicone objective, and a new stage to hold slides, dishes and multi-well plates. Finally we have invested in a fully environmentrally controlled setup for live imaging.
July 25, 2018
Our new high-content system Celldiscoverer 7 has been installed, successfully tested and is now available for usage. It represents an “all-in-a-box” system, dedicated for long-term and high-content imaging. The major new technical features include automatic detection of sample containers and measurement of specimen-specific physical parameters (RI, bottom composition and thickness). The latter are used to adjust auto-corrected objectives to allow optical optimization for the respective setup. An intricate focus detection hierarchy allows the user to programme easy to very complex imaging routines.