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Bacteria live under ever-changing environmental conditions. To survive and propagate, bacteria sense environmental cues and respond to them quickly allowing for adaptation. Our research aims to unravel novel principles and pathways underlying signal-perception, -transduction and cellular regulation in the model organism Escherichia coli and related bacteria including pathogens. We focus on mechanisms acting at the post-transcriptional level including small RNAs and RNA-binding proteins. Moreover, we study two-component and phosphotransferase systems, which monitor the environment and transduce information into the cell. Specifically, we investigate a regulatory network, which controls cell envelope biosynthesis and repair in response to metabolic signals and extracytoplasmic stress. This network involves the trans-envelope signaling complex QseEGF, which employs a regulatory cascade composed of small RNAs GlmY/GlmZ and the RNA binding protein RapZ to achieve envelope homeostasis.
We are bacterial geneticists and use the whole repertoire of molecular biology methods to address our questions. We are devoted to the power and art of genetic screens that often reveal novel regulatory pathways and factors. For our RNA-based research projects we employ classical methods such as Northern, EMSA or RACE analyses as well as state-of-the-art approaches including RNA-seq, RNA structure probing and pull-down techniques to analyze RNA/protein interactions. Furthermore, the laboratory has a longstanding expertise in analyzing protein phosphorylations and protein-protein interactions using a variety of complementary in vivo and in vitro techniques. Through application of ligand fishing approaches we routinely identify novel interaction partners for our proteins of interest. Structural and biophysical techniques and bioinformatics are applied in the framework of established collaborations.
Boris Görke studied Biology and obtained a PhD at the University of Freiburg, Germany. Boris performed a postdoc at the Laboratoire De Chimie Bacteriénne, CNRS in Marseille, France. Subsequently he moved to the University of Göttingen, Germany where he started his own research group and received the venia legendi for “Microbiology and Genetics”. Since 2013 he is group leader at the Max Perutz Labs
We recently revealed a conserved signaling cascade acting across three different cellular compartments. The outer-membrane lipoprotein QseG activates the inner membrane-bound kinase QseE by interaction to phosphorylate a cytoplasmic response regulator. This three-component system senses a stimulus, likely derived from the cell envelope, to activate genes required for envelope synthesis and repair.
Kinase KdpD can be activated by non-phosphorylated PtsN, a component of the PTS(Ntr), resulting in phosphorylation of response regulator KdpE. Paradoxically, PtsN and KdpE compete for binding as both contact the KdpD transmitter domain. We found that PtsN binds one protomer in the KdpD dimer to stimulate phosphorylation of the second protomer in trans, which finally phosphorylates KdpE.
The RNA-binding protein RapZ is part of a regulatory circuit involving the two homologous small RNAs GlmY and GlmZ, which jointly regulate synthesis of cell envelope precursor molecules. Our recent work on RapZ revealed the structure and features of a novel type of an RNA-binding protein, which allosterically activates endoribonuclease RNase E by interaction to selectively degrade GlmZ on demand.
We recently discovered that small RNAs GlmY and GlmZ provide Enterobacteria including important pathogens with intrinsic resistance towards antibiotics targeting the central cell envelope synthesis enzyme GlmS. Thus, suppression of the GlmY/GlmZ circuit increases antibiotic susceptibility, which could be useful to treat infections and may help to overcome the current antibiotic resistance crisis.
Two Small RNAs Conserved in Enterobacteriaceae Provide Intrinsic Resistance to Antibiotics Targeting the Cell Wall Biosynthesis Enzyme Glucosamine-6-Phosphate Synthase.
Khan MA, Göpel Y, Milewski S, Görke B.
Domain swapping between homologous bacterial small RNAs dissects processing and Hfq binding determinants and uncovers an aptamer for conditional RNase E cleavage.
Göpel, Yvonne; Khan, Muna Ayesha; Görke, Boris
Targeted decay of a regulatory small RNA by an adaptor protein for RNase E and counteraction by an anti-adaptor RNA.
Göpel, Yvonne; Papenfort, Kai; Reichenbach, Birte; Vogel, Jörg; Görke, Boris
Project title: “Signal perception and transduction by small RNAs GlmY and GlmZ" (P26681-B22)”
Our research on small RNAs is supported by the FWF.
The Group Görke participates in the special Doctoral Program "RNA Biology" reviewed and funded by the Austrian Research Fund FWF.
The Görke group participates in the Special Research Area (SFB) "RNA-Reg - RNA regulation of the transcriptome" funded by the Austrian Science Fund FWF. SFB's are peer-reviewed, highly interactive research networks, established to foster long-term, interdisciplinary co-operation of local research groups working on the frontiers of their thematic areas.
From planarians to parasites: unexpected roles for monoamine transmitters
Speaker: Phil NewmarkInstitute: University of Wisconsin-MadisonHost: Elly Tanaka
Structural guided nanobodies against covid19 and tomography in a box
Speaker: Jim NaismithInstitute: The Rosalind Franklin Institute, OxfordshireHost: David Haselbach
The Birth and Death of Macromolecular Complexes in Eukaryotic Evolution
Speaker: Berend SnelInstitute: Utrecht UniversityHost: Fred Berger
Probing chromosome mechanics in the interphase nucleus
Speaker: Antoine CoulonInstitute: Institut CurieHost: Anton Goloborodko and Daniel Gerlich
Monday Seminar (Internal)
Speaker: JONASSON Mattias (Tanaka), HOHMANN Ulrich (Brennecke/Plaschka), MANOLOVA Toni (Falk)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
The Earth BioGenome Project: Progress Toward a New Synthesis in Evolution Science
Speaker: Harris LewinInstitute: UC DavisHost: Darrin Schultz and Oleg Simakov
Is cohesin necessary for transcription? Molecular entities of transcriptional dysregulation in the absence of cohesin revealed by analysis of transcription elongation complexes
Speaker: Katsuhiko ShirahigeInstitute: Karolinska InstituteHost: Franz Klein
Monday Seminar (Internal)
Speaker: WU Shuangyang (Dolan), SCARAMUZZA Federico (Tessmar), KROGULL Daniel (Burga)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
The recently discovered ties between teeth and infants in human evolution
Speaker: Leslea HluskoInstitute: CENIEHHost: Nicole Grunstra
Characterisation of Vpr1, a novel protein involved in DSB repair
Speaker: Mustafa AlaaboInstitute: Dept. Chromosome Biology, Univ. ViennaHost: Franz Klein
Title to be announced
Speaker: Roberto CerbinoInstitute: University of ViennaHost: Arthur Sedivy
Marine symbioses fueled by wood
Speaker: Dan DistelInstitute: Ocean Genome Legacy CenterHost: Jillian Petersen
New insights in gene regulation and chromatin folding by enhancers and cohesin
Speaker: Wouter de LaatInstitute: Hubrecht InstituteHost: Christa Bücker
Molecular mechanism analysis involved in mitochondrial sheath formation and spermiation processes using gene-manipulated mice
Speaker: Keisuke ShimadaInstitute: Research Institute for Microbial Diseases, Osaka UniversityHost: Andrea Pauli
Monday Seminar (Internal)
Speaker: JELENIC Stela (Saha), ABDRAKHMANOV Alibek (Dagdas), GALUSKA Philipp (Djinovic)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Title to be announced
Speaker: Christoph KreutzInstitute: University of InnsbruckHost: Georg Kontaxis
Microbial trait-based scaling from single populations to communities with consequences for ecosystem processes
Speaker: Ashish MalikInstitute: University of AberdeenHost: Andreas Richter
How chromosome architecture shapes brain architecture
Speaker: Daniele CanzioInstitute: UCSFHost: Anton Goloborodko
Monday Seminar (Internal)
Speaker: MAYER Rupert (Proteomics Tech Hub), WILLIAMS Thomas (Clausen), GEISMANN Maximilian (Balzarotti)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Comprehensive and quantitative characterization of aneuploidy phenotypes
Speaker: Manuela Sophie KollerInstitute: Dept. Chromosome Biology, Univ. ViennaHost: Chris Campbell
Cellular crosstalk in Neurodevelopmental Disorders
Speaker: Silvia CappelloInstitute: MPI of Psychiatry MunichHost: Jürgen Knoblich
Top down and bottom up exploration of human gut microbial communities
Speaker: Karoline FaustInstitute: KU LeuvenHost: Lucia Fuchslueger
Title to be announced
Speaker: Chris OostenbrinkInstitute: BOKU WienHost: Kristina Djinovic-Carugo
Monday Seminar (Internal)
Speaker: PACHECO FIALLOS Franics Belen (Plaschka), FONTES OLIVEIRA Tiago Manuel (Penninger)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
The Mirage of Biosocial Complexity: Critique and Collaboration Around the Tools of Epigenetics
Speaker: Luca ChiapperinoInstitute: Institute of Social Sciences (ISS), University of LausanneHost: Vienna Open Lab
Monday Seminar (Internal)
Speaker: KHALDIEH Nina (Ameres), LORENZO ORTS Laura (Pauli), ROSS James (Burga)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Title to be announced
Speaker: Samer HattarInstitute: NIHHost: Kristin Tessmar
Monday Seminar (Internal)
Speaker: RAFFL Gerald (Ameres), SAJTOS Bettina (Zuber), LANGER Christoph (Gerlich)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Title to be announced
Speaker: Anshul KundajeInstitute: Stanford UniversityHost: Alex Stark
Monday Seminar (Internal)
Speaker: STEINACKER Thomas (Gerlich), EDER Stephanie (Zimmer), TIKANOVA Polina (Burga)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: SCHELLHAAS Ulla (Plaschka/Ameres), RUSCH Emma (Goloborodko), VULIN Milica (Obenauf)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: RADHAKRISHNA PILLAI Balashankar (Saha), MIGOTTI Mario (Konrat), RÖHNSER Josef (Pauli)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: BELL Caelan (Gerlich), MEREITER Stefan (Penninger), BINDL Michael (Leeb)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: LORBEER Franziska (Stark), MULVEY Hugh (Dolan), PÜHRINGER Florian (Burga)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: HOLZER Elisabeth (Martens), COLOZZA Gabriele (Koo), FALCON CHAVEZ Francisco (Tanaka)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: BLAHA Andreas (Pauli), OKUN Anastasia (Karagöz), JUNG Pauline (Obenauf)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Title to be announced
Speaker: Sir Philip CohenInstitute: University of DundeeHost: Egon Ogris
Monday Seminar (Internal)
Speaker: LICHT-MAYER Simon (Penninger), CAYGILL Samuel (Dolan), ADELMANN Leonie (Raible)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Title to be announced
Speaker: Feng ShaoInstitute: National Institute of Biological Sciences, BeijingHost: Pavel Kovarik
Monday Seminar (Internal)
Speaker: SHUNMUGAM Dhanlakshmi (Baccarini), CECI GINISTRELLI Lavinia (Mendjan), VORLÄNDER Matthias (Plaschka)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Title to be announced
Speaker: Jan LöweInstitute: MRC Laboratory of Molecular BiologyHost: Jan-Michael Peters
Monday Seminar (Internal)
Speaker: KOK Jian Yi (Berger), GROH Roan (Dagdas), SODERHOLM Adrian (Versteeg)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Title to be announced
Speaker: Stephan GruberInstitute: University of LausanneHost: Franz Klein
Title to be announced
Speaker: Lydia FinleyInstitute: Memorial Sloan Kettering Cancer CenterHost: Johannes Zuber
Monday Seminar (Internal)
Speaker: STRAVS Ana (Knoblich), SANCHEZ DE MEDINA HERNANDEZ Victor (Dagdas), HORENKAMP Simone (Tanaka)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: PROTELL I DE MONTSERRAT Julia (Brennecke/Plaschka), NAGUMO WONG Sakurako (Knoblich), CORIC Aida (Tessmar)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Title to be announced
Speaker: Nancy KlecknerInstitute: Harvard UniversityHost: Franz Klein
Title to be announced
Speaker: Vera Gorbunova and Andrei SeluanovInstitute: University of RochesterHost: EDI group at GMI/IMBA/IMP
Monday Seminar (Internal)
Speaker: GRABARCZYK Daniel (Clausen), TAVERINI Katherina (Urban), MANCHENO JUNCOSA Estela (Mendjan)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Title to be announced
Speaker: Edward LemkeInstitute: IMB MainzHost: David Haselbach
Monday Seminar (Internal)
Speaker: ATTRILL Sarah (Dolan), UIJTTEWAAL Esther (Elling), SCIBISZ Grzegorz (Karagöz)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: DOLESCHALL Balint (Knoblich), FENG Sonjie (Bücker), BOURGUET Pierre (Berger)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Title to be announced
Speaker: Rotem SorekInstitute: Weizmann Institute of Science, RehovotHost: Julius Brennecke
Monday Seminar (Internal)
Speaker: KRUMWIEDE Luisa (Gaidt), KAPRAL Thomas (Zagrovic)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: KOBAKHIDZE George (Dong), ANGELOPOULOU Eleni (Ellis), PACHANO Tomas (Stark)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Title to be announced
Speaker: Stephan GrillInstitute: MPI of Molecular Cell Biology and GeneticsHost: Clemens Plaschka
Monday Seminar (Internal)
Speaker: RAMIREZ America (Köhler), VÖLKL Isabella (Bücker), HOANG Oi Pui (Technau)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Title to be announced
Speaker: Mounia LaghaInstitute: Institut de Genetique Moleculaire de MontpellierHost: Andrea Pauli
Monday Seminar (Internal)
Speaker: LEITNER Moritz (Martinez), VOGEL Alexander (Yudushkin), KETTEL Paulina (Karagöz)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Title to be announced
Speaker: Martin HetzerInstitute: IST AustriaHost: Jürgen Knoblich
Monday Seminar (Internal)
Speaker: TSAREV Aleksandr (Ameres), FIESELER Charlie (Zimmer), ABESAMIS Kim Ivan (Dong)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Title to be announced
Speaker: Laurent NguyenInstitute: University of LiegeHost: Noelia Urban
Monday Seminar (Internal)
Speaker: HEIDARI KHOEI Heidar (Rivron), KLEIFELD Justus (Urban), ORLIC Lucija (Matos)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Title to be announced
Speaker: Ian HicksonInstitute: University of CopenhagenHost: Joao Matos
Monday Seminar (Internal)
Speaker: SHULKINA Alexandra (Versteeg), GEETHA Sowmya (Jantsch), MADHUSUDHANAN Jalaja (Zimmer)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: KOLLER Manuela (Campbell), GUPTA Ankit (Goloborodko), SCHMÖLLERL Johannes (Zuber)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: MARCHIORI Elisa (Van der Veeken), ACHLEITNER Anja (Martens), FAAS Victoria (Clausen)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: SALEWSKIJ Kirill (Penninger), KIRCHGATTERER Paul (Gaidt), REN Kaike (Otsuka)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: MIHAILOVIC Milica (Karagöz), BELL Lillie (Clausen), TELONI Federico (Gerlich)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: STANKUNAS Edvinas (Köhler), LEV Itamar (Zimmer)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: OBERHUEMER Michael (Leeb), GONAZLEZ ALVARADO Maria (Grade), SCHOLZ Julia (Otsuka)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: AMANN Sascha (Haselbach), KASHKO Nataliia (Matos), BAUER Bernd (Martens)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: LEE Joonsun (Elling), LEYVA GONZALEZ Marco (Tanaka), MILIVOJEV Nadja (Raible)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: GAO Peng (Dagdas), GOGOVA Rebeca (Clausen), PAVLOVA Anzhela (Matos)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: AYALA MUNOZ Farja (Plaschka), VETRANO Pamela (Ramundo), YU Changwei (Brennecke)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: KONSTANTINOU Andri (Obenauf), NAVA GARCIA Marintia (Dagdas), WOLFF Uyen Le Phuong (Jantsch V.)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: HARTMANN Felix (Matos), PASSERA Alessandro (Balzarotti), SANCHEZ BURGOS Laura (Obenauf)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: SILVA Raquel (Foisner), HODAKOVA Zuzana (Haselbach), MYLARSHCHIKOV Dmitry (Gerlich)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Title to be announced
Speaker: Johannes WalterInstitute: Harvard Medical SchoolHost: Jan-Michael Peters
Monday Seminar (Internal)
Speaker: PIECH Lucas (Leonard), DE LA CONCEPCION Juan Carlos (Dagdas), POPOVA Mariia (Peters)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: GALLI Lucrezia (Tanaka), DIAS Ana Beatriz (Slade), TAKACS Zsuzsanna (Gerlich)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: KNIEST Judith (Haselbach), KELLNER Max (Penninger), BABADEI Olga (Decker)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: BOHAUMILITZKY Lena (Pinheiro), RAIDERS Stephan (Tanaka), PRLESI Ines (Gerlich)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: ZHAN Ni (Dagdas), PITASI Mattia (Leeb), HEINZL Monika (Stark)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: VELETI Lalitha (Pauli), ENGL Magdalena (Slade), NAAS Julia (Van Haeseler)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: FARAWAY Rupert (Plaschka), GRUNDMANN Lorenz (Haselbach), KLEINWAECHTER Ava (Slade)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: RÖTZER Johannes (Dolan), MIOCIC-STOSIC Fran (Zagrovic), RITSCHKA Birgit (Tanaka)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: PUSHPA BOSE Greeshma (Urban), RAINA Shiviya (Dammermann)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: COUTURIER Jeanne (Jachowicz), PABST Gabriel (Zuber), ROFFAY Chloe (Pinheiro)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: RUSSETT Colleen (Berger), NAJM Ramsey (Knoblich), HOLLAUS David (Gaidt)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: SHARMA Sanskriti (Ellis), JANKOWSKA Julia (Ameres), PAPAREDDY Ranjith (Dagdas)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: MAURER Eric (Peters), BHARDWAJ Vishakha (Schlögelhofer), DIEZMA Laura (Nordborg/Mari-Ordonez)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: GUZMAN PEREZ Sebastian (Martinez), LITTLEBOY Jamie (Knoblich), RIABOV Daria (Plaschka)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: ZEILER Christina (Kovarik), DOCAVO GARCIA Antonio (Jachowicz), Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: ZMAJKOVIC Jakub (Zuber), DEL CHIARO Alessia (Dagdas), ANTONIOLI Sumire (Leonard)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: HUETTER Christiane (Menche), KRACHT Laura (Knoblich), SANDRU Ana Maria (Baccarini)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: BENEDUM Johannes (Slade), KALIS Robert (Zuber), PATEL Rajvi (Matos)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs
Monday Seminar (Internal)
Speaker: PROTSENKO Liudmila (Brennecke), SHETTY Kavya (Hein), BASSAT Elad (Tanaka)Institute: Open only to IMP/IMBA/GMI/Max Perutz Labs