At the Max Perutz Labs, and within the Vienna BioCenter, the Karagöz and Martinez Labs are joining forces to study how RNA processing enzymes dictate RNA fate during cellular stress.
The position is part of the VIP2 Post-Doc Fellowship Program at the Vienna BioCenter.
VIP2 aims to attract creative and committed Post-Docs with passion for science by offering great career support and freedom to pursue innovative and groundbreaking projects.
We have recently revealed the co-translational cleavage of the Xbp1-mRNA and other mRNAs by the endonuclease IRE1 (Acosta-Alvear D., Karagöz GE. Et al, eLife 2018). IRE1 cleaves mRNAs generating 2’, 3’-cyclic phosphates. While IRE1-cleaved XBP1-mRNA is rejoined by the tRNA-LC, the fate of most IRE1-cleaved mRNAs remains largely unknown. We have discovered ANGEL2, the first human enzyme able to hydrolyze RNA2’, 3’-cyclic phosphates, and we showed that over-expression of ANGEL2 is detrimental for the ligation of Xbp1-mRNA exons by the tRNA-LC (Pinto P. et al, Science 2020). Yet, how ANGEL2 action impacts other IRE1-targeted mRNAs remains unexplored.
These results posit several questions for an enthusiastic VIP2 Post-Doc eager to dissect ER-linked RNA processing starting at the molecular and atomic level. What is the fate of IRE1-cleaved mRNAs? How is the interplay between ANGEL2 and other enzymes acting at RNA 3’ ends such as the exosome? How are these processes regulated during ER stress and oxidative stress? The project will involve complementary approaches to tackle these fundamental pathways in cells and in a test tube. Combining high throughput methods with biochemistry, the VIP2 Post-Doc will dissect molecular mechanisms from cellular complexity to atomic resolution.
Successful candidates should
Hold a PhD degree in cell biology, biochemistry or structural biology
For the eligibility criteria of the VIP2 program, please visit: https://training.vbc.ac.at/vip2-post-doc-program
Please contact us by May 15th, 2021.
The VIP2 application deadline is June 15th, 2021 with a start date of January 1st, 2022.