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In order for our cells to stay healthy and functional, they must identify and dispose of damaged and harmful substances. Autophagy (from the Greek word for self-eating) is a process that cells employ to remove this material so that it can be replaced with new and functional parts. During autophagy cellular substances become engulfed by vesicles, the autophagosomes. How autophagosomes form de novo around their cargos is fascinating, yet still enigmatic. At the end of their biogenesis the vesicles fuse with cellular compartments called lysosomes which degrade the cargo. Autophagy was shown to target a variety of components including protein aggregates, organelles and even invading pathogens after they enter the cytoplasm. Not surprisingly, defects in autophagy have been associated with numerous diseases such as neurodegeneration, cancer and uncontrolled infections.
Past research has identified a plethora of factors that are required for autophagy. However, how these factors act together in order to couple the capturing of the cellular material destined for degradation with the formation of autophagosomes is not well understood. Thus, the challenge now is to assign functions and mechanisms to these factors in order to gain a better understanding of how they work together to enable autophagy. We are a multidisciplinary team that focuses on bottom-up approaches to understand how cells form autophagosomes. To this end, we employ biochemical reconstitution, cell biology, light and electron microscopy as well as structural biology approaches. Our long-term goal is to reconstitute autophagy in vitro and compare the outcome to its working in cells. When those two match up we will understand how cells dispose harmful material.
Sascha Martens obtained his Diploma and Doctoral degrees from the University of Cologne in Germany. For his postdoctoral training he moved to the MRC Laboratory of Molecular Biology in Cambridge, UK. In 2009 he established his independent laboratory at the Max Perutz Labs.
We show that the C-terminal domain of FIP200 binds to the p62 cargo receptor promoting the recruitment and activation of the autophagy machinery at ubiquitin condensates. This in turn results in their sequestration within autophagosomes and eventually degradation of the condensates. Structural studies showed that the C-terminal domain of FIP200 is shaped like a claw.
We found that in vitro the autophagy receptor p62 and ubiquitinated substrates spontaneously phase separate into clusters. Mechanistically, this is based on the crosslinking of p62 filaments by the substrates. We further uncover multiple modes of regulation of the clustering reaction that suggest how this process can be integrated into general proteostasis.
We discovered that the yeast cargo receptor Atg19 directly interacts with the E3-like Atg12–Atg5-Atg16 complex via its LIR motifs. In a fully reconstituted system we show that these interactions are sufficient to mediate Atg8 conjugation at the cargo. The recruitment of the E3-like complex to cargo may be conserved since we show that also human cargo receptors bind the ATG5 protein.
We show that the Atg19 cargo receptor contains multiple interaction sites for the Atg8 protein. Atg8 proteins decorate the autophagosomal membrane and collectively these multiple interaction sites for Atg8 bend the membrane around autophagic cargo material. The close apposition of the membrane and the cargo excludes non-cargo material from its delivery into the lysosomal system.
FIP200 Claw Domain Binding to p62 Promotes Autophagosome Formation at Ubiquitin Condensates.
Turco, Eleonora; Witt, Marie; Abert, Christine; Bock-Bierbaum, Tobias; Su, Ming-Yuan; Trapannone, Riccardo; Sztacho, Martin; Danieli, Alberto; Shi, Xiaoshan; Zaffagnini, Gabriele; Gamper, Annamaria; Schuschnig, Martina; Fracchiolla, Dorotea; Bernklau, Daniel; Romanov, Julia; Hartl, Markus; Hurley, James H; Daumke, Oliver; Martens, Sascha
p62 filaments capture and present ubiquitinated cargos for autophagy.
Zaffagnini, Gabriele; Savova, Adriana; Danieli, Alberto; Romanov, Julia; Tremel, Shirley; Ebner, Michael; Peterbauer, Thomas; Sztacho, Martin; Trapannone, Riccardo; Tarafder, Abul K; Sachse, Carsten; Martens, Sascha
Mechanism of cargo-directed Atg8 conjugation during selective autophagy
Fracchiolla, D., Sawa-Makarska, J., Zens, B., de Ruiter, A., Zaffagnini,G., Brezovich, A., Romanov, J., Runggatscher, K., Kraft,C., Zagrovic, B. and Sascha Martens
Oligomerization of p62 allows for selection of ubiquitinated cargo and isolation membrane during selective autophagy.
Wurzer, Bettina; Zaffagnini, Gabriele; Fracchiolla, Dorotea; Turco, Eleonora; Abert, Christine; Romanov, Julia; Martens, Sascha
Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy.
Sawa-Makarska, Justyna; Abert, Christine; Romanov, Julia; Zens, Bettina; Ibiricu, Iosune; Martens, Sascha
Mechanism and functions of membrane binding by the Atg5-Atg12/Atg16 complex during autophagosome formation.
Romanov, Julia; Walczak, Marta; Ibiricu, Iosune; Schüchner, Stefan; Ogris, Egon; Kraft, Claudine; Martens, Sascha
Sascha Martens coordinates the FWF SFB on "Targeted Protein Degradation".
Sascha Martens was awarded the Program Grant from HFSP for a collaborative project together with scientists from Germany, USA and Japan.
Sascha Martens is awardee of a "Consolidator Grant" from the European Research Council ERC.
The Martens Lab was awarded FWF Stand Alone Grants (P25546-B20, P27799-B20, P30401-B21 and P32814-B) to support our research on autophagy.
Sascha Martens is awardee of a "Starting Independent Researcher Grant" from the European Research Council ERC.
Sascha Martens has been elected member of the "Junge Kurie" of the Austrian Academy of Sciences in April 2011
Sascha Martens joins the network of EMBO Young Investigators.