Mass Spectrometry

The Mass Spectrometry Facility provides proteomics services primarily to the research groups of the Max Perutz Labs Vienna and if capacity remains also to external customers. We apply state-of-the-art LC-MS analysis platforms and bioinformatics tools for identifying and quantifying peptides and proteins, including their post-translational modifications. In addition, we offer consultation, training, and capacities for method development to our users to help finding the best analytical solution for a given problem.

Our services comprise the following applications:

• Identification.
  We perform protein and peptide identification including post-translational modifications from low complexity samples to whole cell lysates using a bottom-up proteomics approach.
   
• Quantification.
  Protein or peptide quantification can be performed in many ways. We provide solutions for all major approaches, such as metabolic or chemical labeling, as well as label-free approaches.
   
• Interaction.
  Affinity-purification or proximity-based labeling strategies (e.g. BioID, APEX) are powerful means to identify protein interaction partners. In combination with mass spectrometry one can tap their full potential.

• Cross-linking mass spectrometry.
  Allows to obtain low-resolution structural information of proteins and protein complexes in solution. Please note that this service is only available to groups from Max Perutz Labs, University of Vienna, and Medical University of Vienna.
   
• Intact protein mass analysis.
  We provide a basic determination of intact proteins under denaturing conditions using ESI-TOF-MS. For example for confirming the mass of an expressed protein or monitoring modifications or stability.